Mouse Anti-AIFM2 Recombinant Antibody (clone HR231)
CAT#: ZG-0843F
This product is a mouse antibody that recognizes AIFM2.
Tested Data
Gene Expression
WB
Figure 1 Mouse Anti-AIFM2 Antibody (ZG-0843F) in WB
Western blot analysis using p53 clone HR231 antibody at 1 µg/ml on native H1299 cells (1) and H1299 cells transfected with human p53. Also shown is a graphic representation of the epitope location.
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Subcellular Location
Figure 1 IF staining of human cell line Hep G2
Immunofluorescent staining of human cell line Hep G2 shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/28961/1537_B3_4_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/75576/1861_G7_31_selected.jpg
Normal Tissue
Figure 3 IHC staining of human spleen
Immunohistochemical staining of human spleen shows strong cytoplasmic positivity in cells in red pulp.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/42309/ihc_selected.jpg
Normal Tissue
Figure 4 Testis
Sertoli cells Staining: Low Intensity: Moderate Quantity: <25% Spermatogonia cells Staining: Medium Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/42309/92094_A_4_6.jpg
Normal Tissue
Figure 5 Spleen
Cells in red pulp Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/42309/92094_B_9_4.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (Hep G2)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000042286-AIFM2
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Specifications
- Host Species
- Mouse
- Type
- Mouse IgG2b
- Species Reactivity
- Human, Monkey, Mouse, Rat
- Clone
- HR231
- Applications
- ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry
Product Property
- Purification
- Protein A or G purified
- Storage
- Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.
Target
REVIEWS AND Q&AS
CITATIONS
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Stable Storage
Over six months of storage at -20°C has not resulted in any degradation of this antibody. It is very stable and maintains its reactivity well.
Low Background
Even when total blocking is not present, the antibody exhibits minimal nonspecific binding, making it a reliable choice for our immunofluorescence studies.
Reproducible Outcomes
Several batches of this antibody have been utilized, and the results have always been consistent. It is very repeatable and dependable for our research.
Q&As
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How can I choose the right secondary antibody for my research?
A: Choose a secondary antibody that is unique to the host species of the primary antibody and compatible with the detection method being used, such as biotin, fluorescent dyes, or HRP.
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Is this antibody robust enough to withstand high stress levels during sample preparation?
A: Recombinant antibodies are usually stable, but to make sure they stay that way, handle them cautiously, follow recommended procedures and storage procedures.
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How can I find out how much of an antibody is in a stock solution?
A: Utilizing a spectrophotometer, determine concentration based on absorbance at 280 nm. To use this approach, one has to know the antibody's specific molar extinction coefficient.
View the frequently asked questions answered by Creative Biolabs Support.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# ZG-0843F, RRID: AB_3112067)
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "AIFM2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0610MZ | Mouse Anti-AIFM2 Recombinant Antibody (clone CBLS-096) | ELISA | Mouse IgG1 |
| ZG-0884F | Mouse Anti-AIFM2 Recombinant Antibody (clone Pab122) | IP, FC, IF | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY68 | Anti-AIFM2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY246 | Anti-Mouse AIFM2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY245 | Anti-Human AIFM2 Immunohistochemistry Kit | IHC |
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